yobH [2019-05-21 18:24:45]

C-terminal fragment of the Y family DNA polymerase [protein|A04F698C8836E84991BC875BCB7B1460D8E74A8E|YozK]-[protein|ED0A85A8A5FCD12C99507EB033F88586521A7C47|YobH]

locus: BSU18930

pI: 10.00

mw: 23.06 kDa

protein length: 217 aa

gene length: 655 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.6|DNA repair/ recombination/ based on similarity]

The protein

Protein family

• [SW|DNA polymerase type-Y family] (according to UniProt)

Structure

• [PDB|4DEZ] (from Mycobacterium smegmatis, 24% identity) [pubmed|22868761]

Expression and Regulation

Operons

• genes: [gene|94F2146AC6998CC77CD2E86CE8BE8DC98AEBB9CD|yozL]-[gene|A04F698C8836E84991BC875BCB7B1460D8E74A8E|yozK]-[gene|ED0A85A8A5FCD12C99507EB033F88586521A7C47|yobH]
  description: [Pubmed|16267290]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

  regulation

  • expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]

Biological materials

Mutant

• MGNA-A306 (yobH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/306 NBRP B. subtilis, Japan]
• BKE18930 (Δ[gene|ED0A85A8A5FCD12C99507EB033F88586521A7C47|yobH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTCAGGATTCTCTT, downstream forward: _UP4_TGAGAAGTATCTCAGTTACG
• BKK18930 (Δ[gene|ED0A85A8A5FCD12C99507EB033F88586521A7C47|yobH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTCAGGATTCTCTT, downstream forward: _UP4_TGAGAAGTATCTCAGTTACG

References

• 16267290,27766092,22868761